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Description
Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5’-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis and virion proteins are expressed. The chromosome replicates and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by recombination.
Phage lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is often used. The DNA sequence used to construct a phage alpha restriction map includes these mutations. The map shows enzymes that cut lambda DNA once. Enzymes produced by Fermentas are shown in orange.
GenBank/EMBL Accession Numbers
J02459, M17233, M24325, V00636, X00906.
Features
Isolated from a heat-inducible lysogenic E.coli W3110 strain.
For DNA sequence, sequence analysis and map creation see free on-line tool REviewer™.
Applications
Activity and specificity assays of restriction enzymes.
Preparation of DNA molecular weight standards.
Cloning. |