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说明：
The DualColor Protein Loading Buffer Pack is a complete solution for the preparation of protein samples prior to SDS-PAGE. Many proteins are sensitive to pH changes that result from temperature fluxuations during electrophoresis in Tris buffers. This optimized loading buffer prevents protein degradation during sample heating prior to SDS-PAGE (1) as well as during the electrophoresis run.
The loading buffer contains two tracking dyes; blue (bromophenol blue) and pink (pyronin Y) which allow for tracking the progress of electrophoresis. In addition, the pink dye permits monitoring of protein transfer to the membrane during Western blot procedures.
The DualColor™ Protein Loading Buffer Pack also contains SDS and DTT for complete disruption of all high-order protein structures. The SDS included in the buffer binds to hydrophobic regions of the protein, causing the protein to unfold and giving it a negative charge. DTT breaks disulfide bonds and destroys residual secondary structures. As DTT is prone to oxidation during multiple freeze-thaw cycles it is supplied in separate vials. Glycerol is included in the loading buffer to ensure protein samples remain in the gel wells after loading.
特性：
Reliable–protects proteins from degradation during sample preparation for SDS-PAGE.
Convenient–pink dye transfers on the membrane durng Western blotting procedure and serves as an indicator for gel lanes.
应用：
Preparation of proteins for SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
Tracking gel lanes on the Western blot membrane.
质量控制：
Tested in protein samples preparation prior to SDS-PAGE and subsequent Western blotting.
组分：
•4X Loading Buffer: 0.25 M Tris-HCl (pH 8.5 at 25°C), 8% SDS, 1.6 mM EDTA, 0.024% Pyronin Y, 0.04% Bromophenol blue and 40% glycerol.
•20X Reducing Agent: 2 M DTT. |