说明: 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase. Yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorouracil, which is toxic to cells. Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and E.coli (pyrF). 应用: Detection of URA3 gene expression in yeast. Construction of yeast 2-hybrid libraries (1). Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements and identification of gene mutations (2). 质量控制: Purity of 98%. 注意: •Low solubility in water. •Solubility in dimethylsulfoxide is greater than 150 mg/ml. •Preparation of a 100 mg/ml solution in dime-thylsulfoxide is recommended. |