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5-Fluoroorotic Acid|5-FOA|原装订购

产品编号: R0811     查看说明书
产品名称: 5-Fluoroorotic Acid|5-FOA|原装订购  .0   订购此产品 
供应商: MBI
规格: 1 g
目录价: 1192.905
库存状态: 现货
CAS编号:
应用范围:
种属来源:
相关信息:

说明:
5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase. Yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorouracil, which is toxic to cells. Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and E.coli (pyrF).
应用:
Detection of URA3 gene expression in yeast.
Construction of yeast 2-hybrid libraries (1).
Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements and identification of gene mutations (2).
质量控制:
Purity of 98%.
注意:
•Low solubility in water.
•Solubility in dimethylsulfoxide is greater than 150 mg/ml.
•Preparation of a 100 mg/ml solution in dime-thylsulfoxide is recommended.

保存条件: -20°C
说明书地址: 点击查看详细
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