说明: PageBlue Protein Staining Solution is a ready-to-use solution for fast and sensitive staining of proteins separated in polyacrylamide gels or PVDF membranes. PageBlue is based on the Coomassie Brilliant Blue G-250 dye. PageBlue dye forms colloidal particles allowing proteins to be preferentially stained eliminating the need for laborious, expensive and hazardous gel de-staining procedures. Proteins are stained to an endpoint, therefore over-staining does not occur even after overnight staining. The linear dynamic range of PageBlue™ extends over two orders of magnitude (5-500 ng, see picture below) and is about 10 times more sensitive than traditional Coomassie Brilliant Blue R250. PageBlue™ staining solution does not contain methanol or acetic acid and can be reused up to three times without any loss in sensitivity. 特性: Ready-to-use. Easy-to-use – simply soak the gel in the solution and observe stained bands. No destaining required. High sensitivity – 5 ng of protein can be detected. More sensitive than Coomassie Brilliant Blue R-250. No destaining required. Safe – does not contain methanol or acetic acid. Broad linear dynamic range. Fast staining – 25-40 minute protocol. Cost efficient – can be reused up to 3 times. 应用: Visualization and quantification of proteins separated in 1D, 2D, IEF polyacrylamide gels. Protein staining after transfer onto PVDF membranes. In situ zymography. Compatible with mass spectrometry, silver staining. 质量控制: Tested in staining of the Protein Molecular Weight Marker on SDS polyacrylamide gel. 保存温度: Stable at 4 to 26°C. |