|
说明：
Glycogen is a highly purified polysaccharide derived from oysters. It is an inert carrier which significantly increases the recovery of nucleic acids by alcohol precipitation. Glycogen is insoluble in ethanol and forms a precipitate that traps target nucleic acids. During centrifugation a visible pellet is formed which greatly facilitates handling of the precipitated nucleic acids. Glycogen quantitatively precipitates nucleic acids from diluted solutions with a higher efficiency than that of tRNA, linear polyacrylamide or sonicated DNA (1-4).
Fermentas offers two formulations of aqueous glycogen solutions that allow for fast and efficient DNA or RNA recovery.
Glycogen, molecular biology grade, recommended for DNA precipitation.
Glycogen, RNA grade, can be used for both RNA and DNA precipitation.
特性：
Ideal for high recovery of oligonucleotides (>8 bases) and low amounts of DNA/RNA (>20 pg) from diluted solutions.
Forms clearly visible pellets.
Does not interfere with enzymatic reactions*.
Does not interfere with gel electrophoresis of nucleic acids.
Does not interfere with spectrophotometrical determination of DNA/RNA concentration, (A260-280 measurements).
Compatible with DNA transformation and in vitro transfection.
*At a final concentration up to 8 µg/µl, Glycogen does not interfere with PCR, DNA sequencing, DNA digestion by endonucleases, ligation, cDNA synthesis, DNA labeling, in vitro transcription or bacterial transformation. At a final concentration up to 0.4 µg/µl, Glycogen does not affect in vitro transfection of eukaryotic cells with ExGen 500 in vitro Transfection Reagent.
质量控制：
Absence of endo-, exodeoxyribonucleases, ribonucleases, phosphatases, proteases and nicking activity confirmed by appropriate quality tests. Glycogen, molecular biology grade, is functionally tested for DNA precipitation. Glycogen, RNA grade, is functionally tested for RNA precipitation. |