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说明：
X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see the scheme below).
X-Gal Solution, ready-to-use, is stable, 0.22 µm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.
应用：
Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1).
Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells.
Detection of beta-galactosidase activity in immunological and histochemical procedures.
质量控制：
X-Gal is tested by HPLC. Purity of ≥ 99.0% confirmed. Functionally tested in blue/white cloning assay.
注意：
•Preparation of a 20 mg/ml stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal. DMF dissolves some plastic materials. The direct addition of DMF containing solution to plastic Petri dishes should be avoided.
•DMF is toxic. Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.
保存温度：
X-Gal Solution, ready-to-use is stable for at least 12 months at 4°C or at room temperature in the original dark vial.
X-Gal should be stored at -20°C in the dark. |