说明: PureExtreme® dNTPs are manufactured at Fermentas, one of a few primary manufacturers in the industry. They are supplied in aqueous solutions titrated to pH 7.0 with NaOH. dNTPs are available individually, as a convenient set of 100 mM solutions of each dATP, dTTP, dCTP and dGTP for direct use in PCR, reverse transcription, labeling and other applications. 特性: Greater than 99% purity confirmed by HPLC. Free of human and E.coli DNA. Highly stable – the neutral pH of nucleotide solutions ensures the stability during long-term storage: – stable after multiple freeze-thaw cycles; – 90-95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature; – 85-90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C). Application tested in: – long range PCR (40 kb), – cDNA synthesis and RT-PCR, – real-time PCR, – standard PCR, – high fidelity PCR. 应用: For use in all molecular biology applications including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling and DNA sequencing. 质量控制: •Functionally tested in PCR. •Greater than 99% purity of each component confirmed by HPLC. •Free of endo-, exodeoxyribonuclease, ribonuclease and nicking activities.
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