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GeneJET PCR Purification Kit|现货正品
特性：
Highly efficient: 90-100% recoveries in the range of 100 bp – 10 kb.
Fast: procedure takes only 5 minutes.
Convenient: spin columns are capped and assembled with collection tubes.
说明：
The GeneJET™ PCR Purification Kit is designed for rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures.
The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. The GeneJET™ PCR Purification Kit effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET™ purification column has a total binding capacity of up to 25 µg of DNA and the entire procedure takes just 5 min. The purified DNA can be used in common downstream applications such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.
应用：
Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:
– FastDigest® or conventional restriction digestion;
– automated fluorescent or radioactive sequencing;
– PCR;
– in vitro transcription;
– in situ hybridization;
– labeling;
– cloning.
质量控制：
The kit is tested in the purification of 100 bp and 5.5 kb PCR products according to the protocol. The quality of the purified DNA is evaluated spectrophotometrically, by agarose gel electrophoresis, digestion with FastDigest® restriction enzymes and automated fluorescent sequencing.
成分：
•Binding Buffer
•Wash Buffer (concentrated)
•Elution Buffer (10 mM Tris-HCl, pH 8.5)
•GeneJET™ Purification Columns (preassembled with collection tubes)
•Detailed Protocol |