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PageSilver Silver Staining Kit|现货原装
成分：
•Sensitizer concentrate
•Staining reagent
•Developing reagent
•Formaldehyde
•Stop reagent
•Detailed Protocol
特性：
High sensitivity – 0.05 ng of protein per band can be detected (Fig.2) when the conventional protocol is used.
Clear background (Fig.1).
Fast and easy staining – procedure takes only 60 minutes when the fast protocol is used.
Compatible with in situ zymography.
说明：
The PageSilver Silver Staining Kit is a complete system for extremely sensitive and rapid staining of protein, DNA, RNA in polyacrylamide gels. The procedure is based on the use of silver nitrate to bind proteins at a weakly acidic pH and subsequent reduction of silver ions to metallic silver by formaldehyde at an alkaline pH (1, 2, 3). The staining procedure is fast, simple and can be completed in approximately 60 minutes with very little hands-on time. All components of the PageSilver Silver Staining Kit are optimized for maximum sensitivity of protein staining (Fig.2) with a clear background (Fig.1). As little as 0.05-0.6 ng of protein per band can be visualized. This reflects a >100 times higher sensitivity compared to traditional Coomassie Brilliant Blue R250 staining and allows for superior detection of low abundance proteins.
应用：
Highly sensitive staining of proteins, DNA and RNA in polyacrylamide gels.
Stains both non-denaturing or denaturing polyacrylamide gels containing SDS or urea.
Visualization of nanogram quantities of DNA and RNA.
质量控制：
The kit is tested using the staining protocol for maximum speed, different dilutions of BSA (0.1-2.0 ng) are electrophoresed on a 1.0 mm. 12% Tris-glycine SDS gel. Silver staining detects 0.1 ng BSA in clear background. |