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Biotin DecaLabel DNA Labeling Kit|进口原装
成分：
•Klenow Fragment, exo-
•Decanucleotides in Reaction Buffer
•Biotin Labeling Mix
•Control Template
•Biotin-labeled DNA
•Water, nuclease-free
•Detailed Protocol
特性：
Non-radioactive labeling of DNA.
Efficient priming of labeling reactions with random decamers.
High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction.
说明：
The Biotin DecaLabel™ DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method originally developed by Feinberg and Vogelstein (1, 2). The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. Klenow Fragment, exo- is also included in the kit; this genetically engineered enzyme has no detectable exonuclease activity. Therefore, the enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. As a result, DNA fragments of any length can be uniformly labeled. Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or with conventional biotin-avidin or biotin-streptavidin detection systems.
应用：
Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations.
质量控制:
The kit is tested in a control labeling reaction. The biotin-labeled DNA probe is used in dot-blot. Low amounts of homologous DNA (0.1-0.03 pg) are detected after 16 hours of color development with Biotin Chromogenic Detection Kit.
原理：
Random decamers are annealed to a denatured template DNA molecule and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand (see Fig.1). |