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现货供应|Silica Bead DNA Gel Extraction Kit
成分：
•Silica Powder Suspension
•Binding Buffer
•Concentrated Washing Buffer
•TBE Conversion Buffer
•Detailed Protocol
应用：
Purification of DNA fragments from agarose gels prepared with TAE or TBE buffers.
Concentration of DNA samples for desalting or buffer change.
Removal of enzymes after PCR, restriction digestion, dephosphorylation or labeling reactions.
Removal of unincorporated nucleotides, primers and primer-dimers.
Removal of linkers following ligation.
Removal of residual salts, phenol, chloroform or ethidium bromide.
Purification of RNA-free plasmid DNA.
特性：
Flexible – easy to scale up or down both reaction and elution volumes.
Efficient – greater than 80% DNA recovery.
Fast – purification takes only 15-20 min.
Effective – suitable for DNA fragments as short as 100 bp.
Safe – does not involve phenol extraction.
说明：
The Silica Bead DNA Gel Extraction Kit is a simple and efficient system for DNA extraction from agarose gels and reaction mixtures. The kit utilizes the modified protocol of Vogelstein and Gillespie (1). The extraction is based on the solubilization of the agarose gel and selective adsorption of nucleic acids on specially prepared silica particles at high salt concentration. Silica particles bound with DNA are washed to remove contaminants and pure DNA is eluted with TE buffer or water. This method requires few manipulations, and is both faster and easier to perform than other organic-based extraction methods. Compared to column based gel-extraction kits, the DNA Gel Extraction Kit offers flexibility for scaling up or down reaction and elution volumes. The purified DNA is suitable for all common molecular biology procedures, including restriction digestion, cloning, sequencing, etc.
质量控制：
The kit is functionally tested in the purification of a 950 bp and 120 bp DNA fragment from a 1% agarose gel and from a PCR mixture. |