Long PCR Enzyme Mix|现货正品应用:Long range PCR. PCR from GC-rich or difficult templates. Generation of template for TA cloning.RT-PCR.说明:Long PCR Enzyme Mix is a unique blend of Fermentas Taq DNA Polymerase and a thermostable high fidelity DNA polymerase with proofreading activity. The two enzymes synergistically generate long PCR products with greater yield and fidelity than Taq DNA Polymerase alone. The fidelity of PCR with this enzyme mix is three times higher than with Taq DNA Polymerase. The ratio of enzymes in the Long PCR Enzyme Mix is optimized for generation of very long amplicons: up to 47 kb with viral DNA and up to 21 kb with genomic DNA templates (see Fig.1). The specially formulated Long PCR Buffer protects DNA from depurination and nicking during long thermal cycling. The products generated with the Long PCR Enzyme Mix are mostly 3'-dA tailed. Long PCR Enzyme Mix is also used for efficient amplification of GC-rich DNA regions (see Fig. 2).特性:Unique blend of two thermostable DNA polymerases.Long PCR products:– up to 47 kb with viral DNA as template,– up to 21 kb with genomic DNA as template.Ideal for GC-rich templates up to 85%.Fidelity is three-times higher than with Taq DNA polymerase (see Fig.1).High yield.友情提示:本产品仅用于生命科学研究实验,不作为任何药物及其他用途。