FastDigest SfiI|正品原装

FastDigest SfiI|正品进口
Recommended Reaction Conditions
1X FastDigest buffer or 1X FastDigest Green buffer at 50°C.
1 µl of FastDigest SfiI is formulated to digest up to:
– 1 µg of pUC19 DNA with inserted SfiI recognition sequence in 15 min
– 1 µg of plasmid DNA in 15 min
– 0.2 µg of PCR product in 15 min
– 1 µg of genomic DNA in 15 min, or 5 µg of genomic DNA in 60 min.
Formulation
1 μl of enzyme (1 FDU) cleaves 1 μg of pUC19 DNA with inserted SfiI recognition sequence in 15 min at 50°C in 1X FastDigest Buffer.
Note
For cleavage with FastDigest SfiI at least two copies of its recognition sequence are required.
Isoschizomers
Search for commercial isoschizomers using REsearch™.
Methylation Effects
Dam: never overlaps – no effect.
Dcm: may overlap – cleavage impaired.
CpG: may overlap – cleavage impaired.
EcoKI: never overlaps – no effect.
EcoBI: never overlaps – no effect.
注：本产品仅用于生命科学研究实验，不作为任何药物及其他用途。