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FastDigest AciI (SsiI)|进口原装
Recommended Reaction Conditions
1X FastDigest buffer or 1X FastDigest Green buffer at 37°C.
1 µl of FastDigest AciI (SsiI) is formulated to digest up to:
– 1 µg of lambda DNA in 5 min
– 1 µg of plasmid DNA in 5 min
– 0.2 µg of PCR product in 5 min
– 1 µg of genomic DNA in 5 min, or 5 µg of genomic DNA in 30 min.
Formulation
1 μl of enzyme (1 FDU) cleaves 1 μg of lambda DNA in 5 min at 37°C in 1X FastDigest Buffer.
Compatible Ends
FastDigest AccI (XmiI), FastDigest AclI (Psp1406I), FastDigest BsaHI (Hin1I), FastDigest Bsp119I, FastDigest ClaI (Bsu15I), FastDigest HinP1I (Hin6I), FastDigest Hpall, FastDigest MspI, FastDigest TaqI, AccI (GT^CGAC), AclI, BsaHI (GR^CGCC), Bsp119I, Bsu15I, ClaI, Hin1I (GR^CGCC), Hin6I, HinP1I, HpaII, MspI, NarI, Psp1406I, TaqI, XmiI (GT^CGAC).
Isoschizomers
Search for commercial isoschizomers using REsearch.
Methylation Effects
Dam: never overlaps – no effect.
Dcm: never overlaps – no effect.
CpG: completely overlaps – blocked.
EcoKI: never overlaps – no effect.
EcoBI: never overlaps – no effect.
注：本产品仅用于生命科学研究实验，不作为任何药物及其他用途。 |