FastDigest BspMI (BveI)|正品原装

FastDigest BspMI (BveI)|现货正品
Recommended Reaction Conditions
1X FastDigest buffer or 1X FastDigest Green buffer + 0.5 µM oligonucleotide at 37°C.
1 µl of FastDigest BspMI (BveI) is formulated to digest up to:
– 1 µg of lambda DNA in 15 min
– 1 µg of plasmid DNA in 15 min
– 0.2 µg of PCR product in 60 min
– 1 µg of genomic DNA in 20 min.
Formulation
1 μl of enzyme (1 FDU) cleaves 1 μg of lambda DNA in 15 min at 37°C in 1X FastDigest Buffer.
Note
•At least two copies of FastDigest BspMI (BveI) recognition site are required for efficient cleavage.
•Inclusion of 0.5 µM oligonucleotide with the FastDigest BspMI (BveI) recognition sequence in the reaction mixture significantly improves cleavage of plasmid DNAs, especially of those with a single FastDigest BspMI (BveI) site. Still, a complete cleavage of some substrates by FastDigest BspMI (BveI) is difficult to achieve.
Methylation Effects
Dam: never overlaps – no effect.
Dcm: never overlaps – no effect.
CpG: may overlap – cleavage impaired.
EcoKI: may overlap – effect not determined.
EcoBI: may overlap – effect not determined.
Isoschizomers
Search for commercial isoschizomers using REsearch.
注：本产品仅用于生命科学研究实验，不作为任何药物及其他用途。