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FastDigest Fnu4HI (SatI)|正品现货
Recommended Reaction Conditions
1X FastDigest buffer or 1X FastDigest Green buffer at 37°C.
1 µl of FastDigest Fnu4HI (SatI) is formulated to digest up to:
– 1 µg of lambda DNA in 5 min
– 1 µg of plasmid DNA in 5 min
– 0.2 µg of PCR product in 10 min
– 1 µg of genomic DNA in 5 min, or 5 µg of genomic DNA in 30 min.
Formulation
1 μl of enzyme (1 FDU) cleaves 1 μg of lambda DNA in 5 min at 37°C in 1X FastDigest Buffer.
Isoschizomers
Search for commercial isoschizomers using REsearch.
Note
At least two copies of FastDigest Fnu4HI (SatI) recognition site are required for efficient cleavage.
Compatible Ends
GC^SGC – FastDigest NciI (BcnI), FastDigest ScrFI (Bme1390I), BcnI, Bme1390I, NciI, ScrFI.
GC^WGC – FastDigest MvaI, FastDigest ScrFI (Bme1390I), Bme1390I, MvaI, ScrFI.
Methylation Effects
Dam: never overlaps – no effect.
Dcm: never overlaps – no effect.
CpG: may overlap – blocked.
EcoKI: never overlaps – no effect.
EcoBI: never overlaps – no effect. |