I-SceI 现货进口 Conditions for 100% Activity 1X Buffer Tango™: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA. Incubate at 37°C. Ligation and Recleavage After 50-fold overdigestion with I-SceI, more than 95% of the DNA fragments can be ligated and recut. Storage Buffer I-SceI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 500 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol. |