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SatI (Fnu4HI) 正品现货
#ER1642 1000 u
Lot: ___ Expiry Date: _
5'...G C↓N↑G C...3'
3'...C G↓N↑C G...5'
Concentration: 10 u/μl
Source: Staphylococcus arlettae RFL832
Supplied with: 1 ml of 10X Buffer G
1 ml of 10X Buffer Tango™
Store at -20°C
RECOMMENDATIONS
1X Buffer G (for 100% SatI digestion)
10 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 50 mM NaCl,
0.1 mg/ml BSA.
Incubation temperature
37°C.
Unit Definition
One unit is defined as the amount of SatI required to
digest 1 μg of lambda DNA in 1 hour at 37°C in 50 μl of
recommended reaction buffer.
Dilution
Dilute with Dilution Buffer (#B19): 10 mM Tris-HCl
(pH 7.4 at 25°C) 100 mM KCl, 1 mM EDTA, 1 mM DTT,
0.2 mg/ml BSA and 50% glycerol.
Double Digests
Tango™
Buffer is provided to simplify buffer selection for
double digests. 98% of Fermentas restriction enzymes
are active in a 1X or 2X concentration of Tango™ Buffer.
Please refer to the Fermentas Catalog or go to
www.fermentas.com/doubledigest to choose the best
buffer for your experiments.
1X Tango™
Buffer:
33 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate,
66 mM potassium acetate, 0.1 mg/ml BSA.
Storage Buffer
SatI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C),
100 mM KCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA
and 50% glycerol.
Recommended Protocol for Digestion
• Add:
nuclease-free water 16 μl
10X Buffer G 2 μl
DNA (0.5-1 μg/μl) 1 μl
SatI 0.5-2 μl
• Mix gently and spin down for a few seconds.
• Incubate at 37°C for 1-16 hours.
The digestion reaction may be scaled either up or down.
Recommended Protocol for Digestion of PCR Products
Directly after Amplification
• Add:
PCR reaction mixture 10 μl (~0.1-0.5 μg of DNA)
nuclease-free water 18 μl
10X Buffer G 2 μl
SatI 1-2 μl
• Mix gently and spin down for a few seconds.
• Incubate at 37°C for 1-16 hours. |