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OliI (AleI)现货原装
#ER1632 1000 u
Lot: ___ Expiry Date: _
5'...C A C N N↓N N G T G...3'
3'...G T G N N↑N N C A C...5'
Concentration: 10 u/μl
Source: Oceanospirillum linum 4-5D1
Supplied with: 1 ml of 10X Buffer R
1 ml of 10X Buffer Tango™
Store at -20°C
RECOMMENDATIONS
1X Buffer R (for 100% OliI digestion)
10 mM Tris-HCl (pH 8.5), 10 mM MgCl2, 100 mM KCl,
0.1 mg/ml BSA.
Incubation temperature
37°C.
Unit Definition
One unit is defined as the amount of OliI required to
digest 1 μg of lambda DNA in 1 hour at 37°C in 50 μl of
recommended reaction buffer.
Dilution
Dilute with Dilution Buffer (#B19): 10 mM Tris-HCl
(pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT,
0.2 mg/ml BSA and 50% glycerol.
Double Digests
Tango™
Buffer is provided to simplify buffer selection for
double digests. 98% of Fermentas restriction enzymes
are active in a 1X or 2X concentration of Tango™ Buffer.
Please refer to the Fermentas Catalog or go to
www.fermentas.com/doubledigest to choose the best
buffer for your experiments.
1X Tango™
Buffer:
33 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate,
66 mM potassium acetate, 0.1 mg/ml BSA. |