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AarI
#ER1581 125 u
Lot: ___ Expiry Date: _
5'...C A C C T G C(N)4 ↓...3'
3'...G T G G A C G(N)8 ↑...5'
Concentration: 2 u/μl
Supplied with: 1 ml of 10X Buffer AarI
1 ml of 10X Buffer Tango™
2x25 μl of 50X oligonucleotide (0.025 mM)
Store at -20°C
RECOMMENDATIONS
[1X Buffer AarI] + oligonucleotide* (for 100% AarI digestion)
[10 mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10 mM MgCl2,
100 mM KCl, 0.1 mg/ml BSA] + 0.5 μM of oligonucleotide.
Incubation temperature
37°C.
Unit Definition
One unit is defined as the amount of AarI at which no change
in the fragmentation pattern is observed with further increase
of enzyme. 1 μg of lambda DNA is incubated with the enzyme
for 1 hour at 37°C in 50 μl of recommended reaction buffer.
The cleavage of DNA by AarI is never complete.
Dilution
Dilute with Dilution Buffer (#B19): 10 mM Tris-HCl (pH 7.4 at
25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA
and 50% glycerol.
Double Digests
Tango™
Buffer is provided to simplify buffer selection for double
digests. 98% of Fermentas restriction enzymes are active in a 1X or
2X concentration of Tango™ Buffer. Please refer to the
Fermentas Catalog or go to www.fermentas.com/doubledigest
to choose the best buffer for your experiments.
1X Tango™
Buffer:
33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM magnesium acetate,
66 mM potassium acetate, 0.1 mg/ml BSA. |