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BseMII (BspCNI) 原装现货
Conditions for 100% Activity
[1X Buffer Tango™] + SAM:
[33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA] + 0.01 mM S-adenosylmethionine.
Incubate at 55°C.
Ligation and Recleavage
After 10-fold overdigestion with BseMII, more than 90% of the DNA fragments can be ligated, but none of these can be recut due to the methylation of the recognition sequence by this enzyme (see Note)
Storage Buffer.
BseMII is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol. |