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TasI (Tsp509I)现货正品
Conditions for 100% Activity
1X Buffer B:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA.
Incubate at 65°C. To ensure higher efficiency of digestion, perform the cleavage reaction under paraffin oil.
Ligation and Recleavage
After 50-fold overdigestion with TasI, more than 95% of DNA fragments can be ligated and recut.
Storage Buffer
TasI is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
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