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BstXI
Storage Buffer
BstXI is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with BstXI, approximately 95% of the DNA fragments can be ligated and recut.
Conditions for 100% Activity
1X Buffer O:
50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1 mg/ml BSA.
Incubate at 55°C.
Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours. |