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Bsh1285I (BsiEI)
产地：MBI
货号：ER0891
Storage Buffer
Bsh1285I is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 150 mM KCl, 1 mM DTT, 1 mM EDTA, 0.15% Triton X-100, 0.5 mg ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with Bsh1285I, more than 95% of the DNA fragments can be ligated and recut.
Conditions for 100% Activity
1X Buffer G:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl and 0.1 mg/ml BSA.
Incubate at 37°C. |