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Cfr13I (Sau96I)
产地：MBI
货号：ER0191
Conditions for 100% Activity
1X Buffer Tango:
33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
Cfr13I is supplied in:
10 mM potassium phosphate (pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with Cfr13I, more than 95% of the DNA fragments can be ligated and recut. |