The UltraSNAP™ kit consists of Alkaline Phosphatase Streptavidin, the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate as well as specially optimized PolyBlock™ Blocking Reagent and washing solutions formulated to produce high sensitivity with low background.Streptavidin coupled to alkaline phosphatase binds to the biotinylated probe on the blot. The probe is visualized by the conversion of the DuoLuX™ Chemiluminescent/ Chemifluorescent Substrate to a luminescent and fluorescent product by alkaline phosphatase. The sensitivity is enhanced with the use of the PolyBlock™ Blocking Reagent and wash buffers included in the kit which are specifically designed to minimize background.
