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VECTREX® AAL is used for reversible binding of fucose-labeled nucleic acids. Binding and elution can be performed at physiological pH and salt concentrations. VECTREX® AAL contains Aleuria aurantia lectin and will bind nucleic acids labeled with fucose using either the FastTag® system, or 3’ or 5’ EndTag™ Labeling Systems. Elution is easily accomplished under non-denaturing conditions with L-fucose. The matrix is supplied as a 1:1 suspension in buffer. The binding capacity is at least 25 ng fucose-labeled λ DNA per μl 1:1 suspension.
The VECTREX® matrix is optimized for immobilization of nucleic acids. This matrix consists of particles of a highly cross-linked sugar polymer that has a very large surface area and low nonspecific binding for nucleic acids. Unlike agarose, which may retain small molecules in its pores, the VECTREX® particle will retain molecules only through specific affinity interaction on the surface of the particles. The VECTREX® matrix is dense and sediments readily even without centrifugation. |