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Phaseolus Vulgaris Leucoagglutinin has four “L” subunits and is an excellent, specific marker for tracing efferent neuronal projections.
After iontophoretic injection of PHA-L, the approximate rate of anterograde transport is about 4-6 mm/day, with survival periods of over 18 days having been observed. Once transported, the PHA-L is best visualized for light microscopy with antibody to the lectin, followed by the appropriate VECTASTAIN® Elite ABC kit and DAB. Fluorescent staining can also be used for a faster staining procedure. A complete applications protocol is available upon request. |