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This lectin is a 150,000 molecular weight tetrameric glycoprotein consisting of four disulfide-linked chains, two of about 36,000 daltons and two of about 38,000 daltons. Sambucus nigra lectin binds preferentially to sialic acid attached to terminal galactose in (a-2,6), and to a lesser degree, (a-2,3), linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis.
Fluorescein labeled Sambucus Nigra Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Sambucus Nigra Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Fluorescein labeled Sambucus Nigra Lectin has an excitation at 495 nm and an emission at about 515 nm. |