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This lectin is a 66,000 to 70,000 molecular weight dimer composed of two subunits of about 35,000 daltons. No metal ions seem to be required for binding. Four bands are resolved by isoelectric focusing with isoelectric points between pH 6.7 and pH 7.7. Unlike most lectins, it binds best in mildly acidic conditions. Although this lectin preferentially binds to oligosaccharides containing the galactosyl (b-1,3) N-acetylgalactosamine structure (“T-antigen”), tissue staining patterns are markedly different than those obtained with either peanut agglutinin or Jacalin. Amaranthus caudatus lectin also appears to tolerate sialic acid substitution at the 3 position of galactose in the “T” antigen.
Fluorescein labeled Amaranthus Caudatus Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Amaranthus Caudatus Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Fluorescein labeled Amaranthus Caudatus Lectin has an excitation at 495 nm and an emission at about 515 nm. |