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Caspase-12 Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Fluorescence (Ex/Em 400/505) or fluorescence microscopy
• Sample type- Cell and tissue lysates
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25, 100 assays)
• Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Features & Benefits:
• Simple one-step procedure; takes only 1-2 hours
• Fast and convenient
• Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-12 activity.
Kit components:
• Cell Lysis Buffer
• 2X Reaction Buffer
• ATAD-AFC Substrate
• DTT (1 M)
Description:
Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (max = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |