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T4 DNA Ligase (5 u/μl)
An enzyme which anneals DNA strands together
Product Overview
Alternate Names / Synonyms: DNA Ligase
Gene Symbol: N/A
Accession #: N/A
Gene ID: N/A
Source: E. coli
Appearance: Liquid
Physical Form Description: ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50% glycerol (v/v).
Molecular Weight: N/A
Purity by SDS-PAGE: N/A
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Gel Pack
Background Information: T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |