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Protein Disulfide Isomerase (PDI), human recombinant
Catalyzes disulfide exchange and protein folding
Product Overview
Alternate Names / Synonyms: Protein Disulfide Isomerase, PDI
Gene Symbol: PDIA1
Accession #: P07237
Gene ID: 5034
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and lyophilized with no additives
Molecular Weight: 57.1 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: ≥95%
Endotoxin Level: <0.1 ng/μg
Biological Activity: Recombinant PDI is biological active. Reductase activity is 1.0 x 10e3 ∆650nm/ min-2, by measuring the turbidity increase at 650 nm due to insulin reduction. The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time. Isomerase activity is 0.5 µmol active RNase A min-1 µmol PDI-1, according to the re-activation of reduced and denatured RNase A.
Reconstitution Instructions: Reconstituted PDI should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add carrier protein (0.1% HAS or BSA).
Storage Temp.: -20°C
Shipping: gel pack
Background Information: Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human PDI produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 508 amino acids and having a molecular mass of 57.1 kDa. The human PDI contains N-terminal 6xHis-tag and is purified by proprietary chromatographic techniques.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |