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GSK-3b, human recombinant
A phosphorylating and an inactivating agent of glycogen synthase
Product Overview
Alternate Names / Synonyms: gsk3b,Glycogen synthase kinase-3 (GSK-3), Active GSK-3b
Gene Symbol: GSK3B
Accession #: P49841
Gene ID: 2932
Source: E. Coli.
Appearance: Lyophilized powder
Physical Form Description: Lyophilized from 2 mg/ml in 0.1 M NH4CO3
Molecular Weight: 53.0-56.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: Activity of GSK-3β was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 µg of the recombinant GSK-3β in a 20 µl reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerol phosphate, 12 mM MgCl2, 2 mM DTT, 0.1 M Na3VO4, and 200 µM ATP. After 30 minutes incubation at 30°C, phosphorylation of Tau by the recombinant GSK-3β was determined by Western blot using a phospho-Tau antibody.
Reconstitution Instructions: Reconstitute to 1 mg/ml in PBS containing 15% glycerol. The solution should be stored at –70°C, stable for up to 3 months.
Storage Temp.: -70°C
Shipping: dry ice
Background Information: Glycogen synthase kinase-3 (GSK-3) is a serine/theronine kinase that was first characterized by its role in intermediary metabolism. In mammals, serine kinase is encoded by two related genes called GSK-3α (51 kDa) and GSK-3β (46 kDa). GSK-3 has been implicated in the regulation of the c-Jun/AP-1 transcription factor and other intracellular signaling pathways.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |