|
Protein A/G
Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
Product Overview
Alternate Names / Synonyms: Proteina/g, protein ag, protein a/g, proteinag, protein-ag, protein-a/g, proteinag, Protein A/G, rProtein A/G, r-protein A/G, recombinant protein A/G, IgG purification, antibody purification, Protein A, Protein G, Protein A&G
Gene Symbol: N/A
Accession #: N/A
Gene ID: N/A
Source: E. coli
Appearance: Lyophilized
Physical Form Description: Lyophilized powder
Molecular Weight: 59.7 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: N/A
Reconstitution Instructions: Reconstitution in water to a concentration of 5 mg/ml gives a clear solution.
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |