Protein A

Protein A (Lyophilized)
Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
Product Overview
Alternate Names / Synonyms: proteina, protein-A, protein a, protein/a, Protein A, rProtein A, r-protein A, recombinant protein A, IgG purification, antibody purification, Protein A beads, Protein A sepharose, Protein A Sepharose Beads
Gene Symbol: N/A
Accession #: YP_498670
Gene ID: 3919448
Source: E. coli
Appearance: Lyophilized
Physical Form Description: Lyophilized powder
Molecular Weight: 39.21 kDa
Purity by SDS-PAGE: ≥97%
Purity by HPLC: ≥97%
Endotoxin Level: <0.1 ng/μg
Biological Activity: N/A
Reconstitution Instructions: Reconstitute in water
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus and C-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.