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His-Tag Antibody
Product Overview
Antibody Target: His-Tag
Alternative Name: His tag, HHHHHH tag
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: 6xHis synthetic peptide
Accession#: N/A
Gene ID: N/A
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification: Affinity purified
Species Reactivity: all
Application: Western blot, Immunoprecipitation/IC
Application & Usage: Western blotting (1 µg/ml), immunoprecipitation (40 µg/ml), and immunocytochemistry (2 µg/ml). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |