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HA-Tag Antibody (Clone 12CA5[2])
Product Overview
Antibody Target: HA-Tag
Alternative Name: GRIN2A , hNR2A , NMDAR2A , NR2A
Host: Mouse
Antibody Type: Monoclonal (Clone 12CA5[2])
Isotype: Mouse IgG2b
Immunogen: Synthetic peptide (YPYDVPDYA) derived from the human influenza hemaglutinin protein
Accession#: ADG21028
Gene ID: 295441961
Appearance: Colorless liquid
Concentration: 1.0 mg/ml
Formulation: 200 µg (1 mg/ml) anti-HA-Tag monoclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification: Affinity purified
Species Reactivity: all
Application: Western blot, Immunoprecipitation, Immunofluorescence
Application & Usage: Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected mammalian cells.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |