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Myc-Tag Antibody
Product Overview
Antibody Target: Myc-Tag
Alternative Name: MYC , C-MYC , c-Myc
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 413 of human c-Myc
Accession#: P01106
Gene ID: 4609
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Myc-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification: Affinity purified
Species Reactivity: all
Application: Western blot, Immunoprecipitation, Immunofluorescence
Application & Usage: Western blotting (1-2 µg/ml), immunoprecipitation (20-40 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect endogenous levels of the transcription factor c-Myc.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |