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Bax Antibody
Product Overview
Antibody Target: Bax
Alternative Name: 581, BAX, 600040, P55269, Bax zeta
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to amino acids near the N-terminus of human Bax
Accession#: L22473
Gene ID: 581
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-Bax polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat, monkey
Application: Western blot, Immunoprecipitation
Application & Usage: Western blot analysis (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually.The antibody detects a 20 kDa protein, corresponding to the apparent molecular weight of Bax on SDS-PAGE immunoblots.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: The Bcl-2 (B-cell leukemia/lymphoma 2) family proteins function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bak) apoptosis. Bax contains the conserved Bcl-2 homology 1 (BH1) and 2 (BH2) domains that allow for its homodimerization or heterodimerization with Bcl-2. When Bcl-2 is in excess, apoptosis is inhibited. However, if Bax levels increase in response to a death signal, the cell is pushed toward death.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |