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Bad Antibody
Product Overview
Antibody Target: Bad
Alternative Name: BBC6, BCL2L8, Bcl-2-binding component 6, Bcl-2-like 8 protein
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide surrounding amino acid 115 of mouse Bad
Accession#: Q61337.1
Gene ID: 2493288
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) antigen affinity purified rabbit anti-Bad polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat, monkey
Application: Western blot, Immunoprecipitation, Immunohistochemistry
Application & Usage: Western blot analysis (1-2 µg/ml), immunoprecipitation (4-8 µg/ml), and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the 22 kDa Bad protein and it does not cross-react with related family members.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: The Bcl-2 (B-cell leukemia/lymphoma 2) family proteins function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bad) apoptosis. Similar to other family member, Bad also exhibits sequence homology to Bcl-2. Bad can selectively dimerize with Bcl-xL and Bcl-2, but not with Bax, Bcl-xs, Mcl-1, or itself. In mammalian cells, Bad binds more strongly to Bcl-xL than Bcl-2. This may explain why Bad reverse the death repressor activity of Bcl-xL, but not that of Bcl-2
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |