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产品名称：BRILLIANCE E. COLI / COLIFORM SELECTIVE AGAR
供应商：Oxoid
货号：CM1046
规格：500g
产品说明：
Directions
Suspend 28.1g of Brilliance E. coli/coliform Selective Agar in 1 litre of distilled water. Bring the medium gently to the boil, to dissolve completely. Either pour the medium into sterile Petri dishes or keep molten at 45ºC for pour plate technique.
Storage conditions and Shelf life
Dehydrated medium: store tightly capped in the original container at 10-30°C
Prepared medium will be stable for up to 2 weeks when stored at 2-8ºC
Appearance
Dehydrated medium; light coloured, free-flowing powder
Prepared medium; light straw coloured, transparent gel
Technique
Brilliance E. coli/coliform Selective Agar may be used for the detection and enumeration of Escherichia coli and coliforms in food and water samples.
Prepare food samples by diluting 1:5 or 1:10 (as appropriate) with 0.1% (w/v) sterile Peptone Water (CM0009), and homogenise in a Stomacher or a laboratory blender. Heavily contaminated water samples should first be diluted in Ringers Solution (BR0052) or Maximum Recovery Diluent (CM0733) so that the number of colonies to be counted is of a readable number e.g. 20-100 colonies. Potable water should be concentrated either by centrifugation or by using the filter membrane method. |